Natural products, including medicinal plants, have drawn a lot of interest because of their ability to fight infections that are resistant to drugs. The present study was aimed at investigating the phytochemical and in vitro antimicrobial activities of the ethanolic extract of Vernonia amygdalina (bitter leaf) on Staphylococcus aureus. Standard procedures were used to carry out the phytochemical screening. The agar-well diffusion method was employed to ascertain the plant extract's antibacterial activity. Using a transparent metre rule, the diameter zone of inhibition of the extract against the bacterial isolates was found. Flavonoids, alkaloids, tannins, saponins, and cardiac glycoside were found in the extract as determined by the qualitative and quantitative phytochemical screening. Alkaloids made up the majority of the constituents, accounting for about 11.00%, while flavonoids, saponins, and tannins made up 10.00, 6.00, and 2.90%, respectively, and cardiac glycoside was the least common, making up 1.67%. The result shows the extract's effectiveness against S. aureus. At the highest extract concentration level of 200 mg/ml, the highest activity (11.3 ± 1.14 mm) was seen, whereas the lowest activity was recorded at the lowest concentration (12.25 mg/ml). It is determined that the antibacterial properties of V. amygdalina may be attributed to its potential bioactive phytochemicals. It is concluded that V. amygdalina has potential bioactive phytochemicals that are responsible for its antibacterial activities.
antibacterial activities, extract, phytochemical, Staphylococcus aureus, Vernonia amygdalina, zone of inhibition
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